Hasim, Arafik Lamadi, Rully Tuiyo
Manggabai fish (Glossogobius aureus) is a local fish from Lake Limboto with economic value. In line with the damage to the lake's ecosystem, the pressure on local fish is increasing. The diminishing population of manggabai shows this over time. In general, the local community views manggabai as an endemic fish. Manggabai conservation efforts need to be analyzed from the DNA aspect. This study aimed to explore the identity of manggabai species, analyze genetic distances and construct phylogenetic trees. Fish samples were collected from Lake Limboto and transported to a laboratory of the Faculty of Fisheries and Marine Sciences of Gorontalo State University, Gorontalo, for morphometric and meristic analyses. Tissue was sampled for DNA barcode analysis, using PCR. DNA sequencing was based on PCR. The analyses show that all sampled manggabai specimens from Limboto Lake are a single species, namely G. aureus. The DNA barcoding results are supported by a similarity test with reference sequences of 99-100%, phylogenetic tree sequences of individual manggabai samples, and individual reference fish sequences showing kinship. © 2023, BIOFLUX SRL. All rights reserved.
Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Negeri Gorontalo, Indonesia
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