Andi Setiawan, Fendi Setiawan, Ni Luh Gede Ratna Juliasih, Widyastuti Widyastuti, Aspita Laila, Wawan A. Setiawan, Fernandy M. Djailani, Mulyono Mulyono, John Hendri, Masayoshi Arai
Secondary metabolites of actinomycetes are a potential source of bioactive compounds in the agricultural sector. This study aimed to determine the fungicidal properties of extracts of marine organism-derived actinomycetes. Actinomycetes were isolated from marine organisms using agar media with 1% colloidal chitin in artificial seawater. Then, the isolates were cultured on liquid media with 1% colloidal chitin in artificial seawater under static conditions for 14 days. The culture was extracted, the fungicide properties were evaluated using the microtiter 96-well plate method, and the influence of inhibition was visualized using apotome and SEM. Finally, the active extract was analyzed using LCMSMS. In the present study, 19 actinomycetes were isolated from marine organisms, and the isolates were examined with regard to their antifungal activities. Of these nineteen isolates, the isolate 19C38A1 was picked out from the rest. Hence, it showed significant control towards F. oxysporum. The prospective strain 19C38A1 was determined to be Kocuria palustris 19C38A1. The extract 19C38A1 was shown to cause damage to cell integrity, indicated by the shrinking form, and inhibited germination in the F. oxysporum; subsequently, the chemical characteristics of the compound produced by the potential isolate 19C38A1 indicated the presence of benzimidazole compounds in the active fraction of C38BK2FA. These results indicate that actinomycetes derived from marine organisms near the coast of Oluhuta, Tomini Bay, Gorontalo, related to strain 19C38A1, are not widely known as sources of valuable fungicides. This preliminary information is important, as it can be used as a basis for further development in the search for fungicides derived from marine actinomycetes. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Department of Chemistry, Faculty of Mathematics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia; Department of Fish Processing, Faculty of Fisheries and Marine Science, Gorontalo State University, Gorontalo, 96128, Indonesia; Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Osaka, Suita, 565-0871, Japan
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock