Asri Silvana Naiu, Maria Yasinta Goo, Nikmawatisusanti Yusuf
Seagrass T. hemprichii can produce secondary metabolite compounds that can be utilized as antibacterials. The objective of the research was to ascertain the yield of T. hemprichii leaf extract using different solvents, identify the phytochemical content of seagrass leaf extract T. hemprichii with different solvents, and determine the antibacterial activity of T. hemprichii leaf extract against Escherichia coli and Staphylococcus aureus bacteria with different solvents by measuring the inhibition zone of those bacteria. The research stages comprised observation, sampling, extraction by maceration using methanol (pa), ethyl acetate (pa) and N-hexane (pa) solvents with a material to solvent ratio of 1:11, as well as phytochemical testing and antibacterial activity testing. The data analysis involved qualitative descriptive and quantitative analysis, conducted using a Completely Randomized Design (CRD) based on the treatment of different solvent types, namely P1 (methanol extract), P2 (ethyl acetate extract) and P3 (N-hexane extract), with repetition three times. The data were analyzed using the analysis of variance (ANOVA) method and tested further by Duncan. The yields of the methanol, ethyl acetate and N-hexane extracts were 12.19%, 3.41% and 2.58%, respectively. The phytochemical tests of the methanol extracts revealed the presence of alkaloids, flavonoids, saponins and tannins, while the ethyl acetate extract demonstrated the presence of alkaloids, saponins, tannins, steroids and triterpenoids. In contrast, the N-hexane extract exhibited the presence of alkaloids and tannins. The results of the bacterial inhibition zone of S. aureus and E. coli from the methanol, ethyl acetate and N-hexane extracts, respectively, were as follows: 5.71 mm (medium) and 7.87 mm (medium), 21.48 mm (very strong) and 19.15 mm (strong), 16.85 mm (strong) and 8.73 mm (medium). © 2025 Author(s).
Departement of Marine and Fishery Technology, Faculty of Marine and Fishery Technology, Universitas Negeri Gorontalo, Gorontalo City, 96128, Indonesia
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