Fahrul Ilham, Safriyanto Dako Agus Bahar Rachman, Muhammad Ihsan Andi Dagong, Lellah Rahim, Yulianty
Kacang goat is a small ruminant native from Indonesia and spread widely in several Southeast Asian countries, and has become part of the world’s biodiversity. Growth Hormone (GH) in goat is a protein secreted from anterior hipophyse by somatrotop cells and its formation process under the control of GH gene. Its main function is to stimulate the growth of bone, muscle and fat metabolism. The purpose of this study is to identify the GH gene polymorphism in Kacang goat with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. A total of 168 blood samples from Kacang goat in two regions of Indonesia (Gorontalo and South Sulawesi province) were extracted by using DNA extraction kit to collect whole DNA genome. Amplification and genotyping of GH gene was performed using PCR-RFLP using the HaeIII restriction enzyme. The results obtained two kinds of genotypes, with the AA genotype frequencies (0.095) and AB (0.904). The frequency of allele A (0.547) and B (0.452) indicate a polymorphism in the GH A781G locus in Kacang goat. The observed (Ho) and expected (He) heterozygosity value were respectively 0.0904 and 0.496. GH alleles distribution in Kacang goat populations were not in Hardy-Weinberg equilibrium. Based on statistical analysis, there is no difference between body size of genotype AA and AB. The results of this study can be used as initial information in formulating a strategy for Kacang goat breeding by utilizing Marker Assisted Selection (MAS). © 2016, Fundacion CIPAV. All rights reserved.
Animal Science Department, Faculty of Agriculture, State University of Gorontalo, Gorontalo Jalan Jenderal Sudirman No 6, Kota Gorontalo, 96111, Indonesia; Animal Production Department, Faculty of Animal Science, Hasanuddin University, Jalan Perintis Kemerdekaan Km 10. Tamalanrea, Makassar, 90245, Indonesia
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