Sitty Ainsyah Habibie, Djumanto, Rustadi
This study aimed to analyze the relationship between otolith diameter and the age and size of albino tilapia (Oreochromis sp.) larvae. Albino tilapia broodstock with a male and female composition of 1:3 was stocked in the spawning pond. Observations of incubated eggs were made every day since stocking. The spawning eggs began to appear on days 6 and 7, then hatched in aquarium tanks. Five larvae were taken every day since hatching from day 1 to day 18, then every two days from day 18 to day 30 for otolith collection. Sagitta otoliths were collected by immersing the fish larvae in a 5.25% NaOCl solution. The otoliths left behind were then glued to a glass object using Buehler cement and evaluated using light microscope. Data recorded were total length, otolith diameter, and number of circles appearing day by day. The results showed otoliths of albino tilapia (Oreochromis sp.) larvae were found to formed daily and very clear during observation so that they can be used to determine the age of fish larvae. The otoliths of albino tilapia (Oreochromis sp.) larvae were initially circular and developed into irregular ovals with jagged edges as they aged. Otolith diameter increased with the age and total length of fish and showed a positive and strong correlation. © 2025 Author(s).
Aquatic Resources Management Department, Faculty of Marine and Fisheries Technology, Universitas Negeri Gorontalo, Gorontalo, 96128, Indonesia; Fisheries Department, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
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