Michihito Sasaki, Gabriel Gonzalez, Yuji Wada, Agus Setiyono, Ekowati Handharyani, Ibenu Rahmadani, Siswatiana Taha, Sri Adiani, Munira Latief, Zainal Abidin Kholilullah, Mawar Subangkit, Shintaro Kobayashi, Ichiro Nakamura, Takashi Kimura, Yasuko Orba, Kimihito Ito, Hirofumi Sawa
Bufavirus is a recently recognized member of the genus Protoparvovirus in the subfamily Parvovirinae. It has been reported that human bufavirus was detected predominantly in patients with diarrhoea in several countries. However, little is known about bufavirus or its close relatives in nonhuman mammals. In this study, we performed nested-PCR screening and identified bufavirus from 12 megabats of Pteropus spp. in Indonesia. Furthermore, we determined nearly the full genome sequence of a novel megabat-borne bufavirus, tentatively named megabat bufavirus 1. Phylogenetic analyses showed that megabat bufavirus 1 clustered with known protoparvoviruses, including human bufavirus but represented a distinct lineage of bufavirus. Our analyses also inferred phylogenetic relationships among animal-borne bufaviruses recently reported by other studies. Recombination analyses suggested that the most common recent ancestor of megabat bufavirus 1 might have arisen from multiple genetic recombination events. These results characterized megabat bufavirus 1 as the first protoparvovirus discovered from megabats and indicates the high genetic divergence of bufavirus.
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan; Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan; Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia; Veterinary Investigation and Diagnostic Center, Bukittinggi, Indonesia; Faculty of Agriculture, Gorontalo State University, Gorontalo, Indonesia; Faculty of Animal Husbandry, Sam Ratulangi University, Manado, Indonesia; Office of Animal Husbandry and Fisheries, Soppeng, Indonesia; Veterinary Medicine Study Program, Faculty of Medicine, Hasanudin University, Makassar, Indonesia; Unit of International Cooperation, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japan; Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Hokkaido, Japan; Global Virus Network, Baltimore, MD, United States; Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan; Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan
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